Title : HIV latency in the humanized BLT mouse 1 Running Title : HIV latency in

INDUCTION OF HIV-1 LATENCY AND REACTIVATION IN PRIMARY MEMORY CD4 T CELLS Running Title: Ex vivo Establishment of HIV-1 Latency

INDUCTION OF HIV-1 LATENCY AND REACTIVATION IN PRIMARY MEMORY CD4 T CELLS Running Title: Ex vivo Establishment of HIV-1 Latency Alberto Bosque and Vicente Planelles Department of Pathology, University of Utah, Salt Lake City, UT 84132 USA Address for correspondence: Vicente Planelles, Ph.D. University of Utah School of Medicine, 15 North Medical Drive East #2100 Room 2520, Salt Lake City, Utah ...

Ultra-Sensitive HIV-1 Latency Viral Outgrowth Assays Using Humanized Mice

In the current quest for a complete cure for HIV/AIDS, highly sensitive HIV-1 latency detection methods are critical to verify full viral eradication. Until now, the in vitro quantitative viral outgrowth assays (qVOA) have been the gold standard for assessing latent HIV-1 viral burden. However, these assays have been inadequate in detecting the presence of ultralow levels of latent virus in a n...

Combinatorial Latency Reactivation for HIV - 1 Subtypes and Variants 1 Running Title : HIV - 1 Combinatorial Anti - latency Strategies

Generation of HIV latency in humanized BLT mice.

Here we demonstrate that a combination of tenofovir, emtricitabine, and raltegravir effectively suppresses peripheral and systemic HIV replication in humanized BLT mice. We also demonstrate that antiretroviral therapy (ART)-treated humanized BLT mice harbor latently infected resting human CD4+ T cells that can be induced ex vivo to produce HIV. We observed that the levels of infected resting hu...

Antiretroviral Pre-exposure Prophylaxis Prevents Vaginal Transmission of HIV-1 in Humanized BLT Mice

BACKGROUND Worldwide, vaginal transmission now accounts for more than half of newly acquired HIV-1 infections. Despite the urgency to develop and implement novel approaches capable of preventing HIV transmission, this process has been hindered by the lack of adequate small animal models for preclinical efficacy and safety testing. Given the importance of this route of transmission, we investiga...